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Scripts for Filtering Raw SNP calls from Affymetrix GeneChip® Resequencing Chips We have developed a set of scripts to identify and remove false positive SNP calls that are present in the CHP files produced by the Affymetrix GSEQ software. These scripts are described in an online presentation available here, and are described more completely in a published manuscript (Nucleic Acids Research 2007; doi: 10.1093/nar/gkm918). These scripts have been designed to work in our processing environment. The download package includes README files, as well as comments in the individual scripts that should help you adapt them to your environment. However the task of adapting these scripts may not be trivial. We offer these scripts "as-is" in the hopes that they will be useful to some in the research community. These scripts require the MUMmer sequence alignment tools, which are available for download here. They also make use of the binding energy calculator from the ArrayOligoSelector package (Bozdech et al, http://genomebiology.com/2003/4/2/R9). The source code for the energy calculator is included with our scripts. Registration not required.



Published on 2021-11-24 09:56:17 - Click here to edit or to add informations - Report as not working

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