PHYSICOCHEMICAL ANALYSIS OF THE PRIMERS
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Category: Physicochemical analysis of the primers
Subcategories: IDT
OligoAnalyzer is a web application from IDT that provides the opportunity to analyze hairpin and self-dimer of existing primers this site. Moreover this site allows you to BLAST the sequence against NCBI's database and measure the impact of incorporating 5'-modifications into the sequence. The oligos can then be ordered directly.
...continue to readOligo Analysis Tool calculates molecular weight (MW), extinction coefficient (E260), pmoles/µg, pmoles/OD and µg/OD. Also displayed for your convenience are the % GC Content, melting temp (TM),the reverse complement of the oligo sequence you enter, and show primer-dimer sets
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